Cat: MF-2021H
Cat: MF-2021H
MIF, Mouse, HEK293 Cells,Tag Free: Product Information
P34884
Human embryonic kidney cell, HEK293-derived mouse MIF protein
Pro2-Ala115
13.3 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Activity in progress
MIF, Mouse, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
MIF, Mouse, HEK293 Cells,Tag Free:Synonyms
GIFmacrophage migration inhibitory factor; GLIF; MMIF; Phenylpyruvate tautomerase
MIF, Mouse, HEK293 Cells,Tag Free:Background
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Mouse MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is
synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (6). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands
interact with other MIF molecules, creating a trimer (2, 8). Structure-function studies suggests MIF is bifunctional with segregated topology. The N- and
C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol- to-keto) has been demonstrated and is dependent upon Pro at
position #1 (9). Amino acids 3-23 have also been shown to be reminescent of a GST glutathione-binding domain (10). MIF has proinflammatory cytokine
activity centered on aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates, NO production and
TNF-alpha release following IFN-gamma activation (11, 12). Mouse MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (13, 14). Mouse MIF is active on human cells, while human MIF is active on mouse cells (12). Mouse MIF is 99%, 84%, 90%, and 90% aa identical to rat,
porcine, bovine and human MIF, respectively.
1.Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
4. Bozza, M. et al. (1995) Genomics 27:412.
5. Mitchell, R. et al. (1995) J. Immunol. 154:3863.
6. Flieger, O. et al. (2003) FEBS Lett. 551:78.
7. Lanahan, A. et al. (1992) Mol. Cell. Biol. 12:3919.
8. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
9. Stamps, S.L. et al. (2000) Biochemistry 39:9671.
10. Blocki, F.A. et al. (1993) Protein Sci. 2:2095.
11. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
12. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
13. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
14. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urology 173:615.
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