Cat: HF-1006
Cat: HF-1006
IL6, Human, HEK293 Cells,Tag Free: Product Information
IL6, B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor
Q75MH2
Human embryonic kidney cell, HEK293-derived human IL-6 protein
Pro29-Met212
21.0 kDa
Solution protein
Dissolved in sterile PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells.
The EC50 for this effect is 0.1-0.5 ng/mL.
IL6, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL6 (Catalog # HF-1006) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cells..
Size-exclusion chromatography of recombinant human IL6 protein (280 nm absorbance)
2 ug/lane protein wasresolved withSDS-PAGEunder non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
IL6, Human, HEK293 Cells,Tag Free:Synonyms
IL6, B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor
IL6, Human, HEK293 Cells,Tag Free:Background
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues (1, 2). Mature human IL-6 is 183 amino acids in length and shares 39% sequence identity with mouse and rat IL-6 (3). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (4-7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6R alpha, triggering IL-6R alpha association with gp130 (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (2). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130 -expressing cells that lack cell surface IL-6 R alpha (2). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (2, 10). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1, 2). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5).
1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
2. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
3. Hirano, T. et al. (1986) Nature 324:73.
4. Kestler, D.P. et al. (1995) Blood 86:4559.
5. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
6. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
7. Alberti, L. et al. (2005) Cancer Res. 65:2.
8. Murakami, M. et al. (1993) Science 260:1808.
9. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
10. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Human IL33 产品展示
Human IL29 产品展示
Human IL28B 产品展示
Human IL28A 产品展示
Human IL27 产品展示
Mouse CCL20 产品展示
Human IL25 产品展示
Mouse IL25 产品展示
Mouse IL23 产品展示
Human IL21 产品展示
Mouse IL21 产品展示
Human IL17A 产品展示
Mouse IL17A 产品展示
Human IL15 产品展示
Human IL13 产品展示
Mouse IL13 产品展示
Human IL12 产品展示
Mouse IL12 产品展示
Human IL11 产品展示
Mouse IL11 产品展示
Mouse CCL2 产品展示
Human IL10 产品展示
Human IL9 产品展示
Human IL8 产品展示
Human IL7 产品展示
Mouse IL7 产品展示
Human IL6 产品展示
Mouse IL6 产品展示
Human IL5 产品展示
Mouse IL5 产品展示
Human IL4 产品展示
Mouse IL4 产品展示
Human IL3 产品展示
Mouse IL3 产品展示
Human IL1-alpha 产品展示
Mouse IL1-alpha 产品展示
Human IL1-beta 产品展示
Mouse IL1-beta 产品展示
Human IL2 产品展示
Mouse IL2 产品展示
