Cat: HF-1004
Cat: HF-1004
IL4, Human, HEK293 Cells,Tag Free: Product Information
Human IL4; hIL-4, recombinant IL4, interleukin 4, BCGF1 Protein
P05112
Human embryonic kidney cell, HEK293-derived human IL4 protein
His25-Ser153
15 kDa
Solution protein
Dissolved in sterile PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The EC50 for this effect is 0.02-0.1 ng/mL.
IL4, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL4 (Catalog # HF-1004) stimulates cell proliferation of the TF-1 human erythroleukemic cells.
Size-exclusion chromatography of recombinant human IL4 protein (280 nm absorbance)
4 ug/lane protein wasresolved withSDS-PAGEunder non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
IL4, Human, HEK293 Cells,Tag Free:Synonyms
Human IL4; hIL-4, recombinant IL4, interleukin 4, BCGF1 Protein
IL4, Human, HEK293 Cells,Tag Free:Background
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a secreted protein that belongs to the IL-4 / IL-13 family (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure (4). Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effectsthrough two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and thecommon γ chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 Rα and IL-13 Rα1.The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). Itpromotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naive CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13).
1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
4. Redfield, C. et al. (1991) Biochemistry 30:11029.
5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Human IL33 产品展示
Human IL29 产品展示
Human IL28B 产品展示
Human IL28A 产品展示
Human IL27 产品展示
Mouse CCL20 产品展示
Human IL25 产品展示
Mouse IL25 产品展示
Mouse IL23 产品展示
Human IL21 产品展示
Mouse IL21 产品展示
Human IL17A 产品展示
Mouse IL17A 产品展示
Human IL15 产品展示
Human IL13 产品展示
Mouse IL13 产品展示
Human IL12 产品展示
Mouse IL12 产品展示
Human IL11 产品展示
Mouse IL11 产品展示
Mouse CCL2 产品展示
Human IL10 产品展示
Human IL9 产品展示
Human IL8 产品展示
Human IL7 产品展示
Mouse IL7 产品展示
Human IL6 产品展示
Mouse IL6 产品展示
Human IL5 产品展示
Mouse IL5 产品展示
Human IL4 产品展示
Mouse IL4 产品展示
Human IL3 产品展示
Mouse IL3 产品展示
Human IL1-alpha 产品展示
Mouse IL1-alpha 产品展示
Human IL1-beta 产品展示
Mouse IL1-beta 产品展示
Human IL2 产品展示
Mouse IL2 产品展示
