CCL2, Human, HEK293 Cells,Tag Free

CCL2, also called monocyte chemotactic protein-1 (MCP-1) or JE, is a member of the C-C or beta  chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature

protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, occurs naturally by

metalloproteinase cleavage and down-regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7-13.5 kDa on SDS-PAGE

due to non-covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78-79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2

can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear

phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2).

In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by

basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2-mediated recruitment of monocytes to

sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7).

1. Yoshimura, T. et al. (1989) FEBS Lett. 244:487.

2. Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.

3. Proost, P. et al.(1998) J. Immunol. 160:4034.

4. Ju Lee, H. et al. (2015) J. Immunol. 194:3634.

5. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.

6. Daly, C. et al. (2003) Microcirculation 10:247.

7. Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.