Cat: HF-4063
Anti-human IgG4 antibody (Secondary), Human, HEK293 Cells,Tag Free: Product Information
This product is sold for research or further manufacturing use only.Standard LaboratoryPractices should be followed when handling this material.
Shipping with dry ice.
-70°C or below is recommended .
Repeated freeze/thaw cycles should be avoided.
Neutral to slightly alkaline pH and h M urea asdissociating agent.
0.4-0.8 ug/mLldepending on the type of ELISA plate, co-coating reagents and coating bufferJ.Suitable for labeling of functional groups.
Standard ELISA test Icheckerboard analysis of positive/negative samples.lmmunodot analyses with positive/negative samples.
27.8 kDa
SDS-PAGE [purity > 95%];
Western blot with i: anti-Tumsatin/GBM antigen autoantibody-positive sample;ii: monoclonal anti-His-tag antibody.
cDNAs coding for the Tumsatin/GBM antigen proteins fused to the histidine purification tag.
Binding lgG-type human auto-antibodies.
Recombinant. Expressed by HEK293 cells
GBM antigens for recurrent anti-GBM disease are the noncollagenous-1 [NC1) domains of the alpha-3 andalpha-5 chains of collagen lV.Antibodies to glomerular basement membrane (GBM] antigens cause glomerulonephritis,Goodpasture syndrome lglomerulonephritis, often with rapid onset renal failure,andpulmonary hemorrhage], and, less commonly, pulmonary hemosiderosis.