Cat: HF-2012
Cat: HF-2012
IGF2, Human, HEK293 Cells,Tag Free: Product Information
P01344.1
Human embryonic kidney cell, HEK293-derived human IGF-II/IGF2 protein
Ala25-Glu91
7.5 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The EC50 for this effect is 0.6-2 ng/mL.
IGF2, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IGF-2 (Catalog # HF- 2012) stimulates cell proliferation of the MCF-7 human breast cancer cells.
Size-exclusion chromatography of recombinant human IGF-II/IGF2 protein (280 nm absorbance)
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IGF2, Human, HEK293 Cells,Tag Free:Synonyms
C11orf43; chromosome 11 open reading frame 43; FLJ22066; GRDF; IGF2; IGF-2; IGFII; IGF-II
IGF2, Human, HEK293 Cells,Tag Free:Background
Insulin-like Growth Factor II (IGF-II) is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth
-promoting function of IGF-II is limited to embryonic development.
Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70%
sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.
Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally
related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation
-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF-II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.
1. Harvey MB, et al. (1991) Development. 111(4): 1057-60.
2. Peters G, et al. (2003) Virchows Arch. 443(2): 139-45.
3. Burrow S, et al. (1998) J Surg Oncol. 69(1): 21-7.
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