Cat: HF-2049
, Human, HEK293 Cells,Tag Free: Product Information
P78556
Human embryonic kidney cell, HEK293-derived human CCL20/MIP-3A protein
Ala27-Met96
8.0 kDa
Solution protein.
Dissolved in sterile PBS buffer .
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR6. The EC50 for this effect is 0.01-1 ng/mL.
, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human CCL20/MIP-3A (Catalog # HF-2049) stimulates cell proliferation of the BaF3 mouse pro-B cells transfected with human CCR2A.
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
, Human, HEK293 Cells,Tag Free:Synonyms
CCL20; chemokine (C-C motif) ligand 20; CKb4; LARCLiver and activation-regulated chemokine;
, Human, HEK293 Cells,Tag Free:Background
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through
bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to
form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity) and the gene for
CCL20 has been mapped to chromosome 2 rather than 17.CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL,
fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the
anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4,
CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord
blood precursors.
1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
2. Hromas, R. et al. (1997) Blood 89:3315.
3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
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