Cat: MF-1007
Cat: MF-1007
IL7, Mouse, HEK293 Cells,Tag Free: Product Information
P10168
Human embryonic kidney cell, HEK293-derived mouse IL-7 proteinGlu26-Ile154
14.9 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The EC50 for this effect is 0.01-0.2 ng/mL.
IL7, Mouse, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL7, Mouse, HEK293 Cells,Tag Free:Synonyms
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
IL7, Mouse, HEK293 Cells,Tag Free:Background
Interleukin-7 (IL-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Mouse IL-7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide (4). Mouse IL-7 shares approximately 88% aa sequence
identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species
activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone
marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive memory T cells, but undergoes IL-7-mediated down-regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is
required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell
development (4, 9, 12, 13).
1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
4. Namen, A.E. et al. (1988) Nature 333:571.
5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
6. Saini, M. et al. (2009) Blood 113:5793.
7. Park, J.H. et al. (2004) Immunity 21:289.
8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
Human IL33 产品展示
Human IL29 产品展示
Human IL28B 产品展示
Human IL28A 产品展示
Human IL27 产品展示
Mouse CCL20 产品展示
Human IL25 产品展示
Mouse IL25 产品展示
Mouse IL23 产品展示
Human IL21 产品展示
Mouse IL21 产品展示
Human IL17A 产品展示
Mouse IL17A 产品展示
Human IL15 产品展示
Human IL13 产品展示
Mouse IL13 产品展示
Human IL12 产品展示
Mouse IL12 产品展示
Human IL11 产品展示
Mouse IL11 产品展示
Mouse CCL2 产品展示
Human IL10 产品展示
Human IL9 产品展示
Human IL8 产品展示
Human IL7 产品展示
Mouse IL7 产品展示
Human IL6 产品展示
Mouse IL6 产品展示
Human IL5 产品展示
Mouse IL5 产品展示
Human IL4 产品展示
Mouse IL4 产品展示
Human IL3 产品展示
Mouse IL3 产品展示
Human IL1-alpha 产品展示
Mouse IL1-alpha 产品展示
Human IL1-beta 产品展示
Mouse IL1-beta 产品展示
Human IL2 产品展示
Mouse IL2 产品展示
