Cat: MF-1012
Cat: MF-1012
IL12, Mouse, HEK293 Cells,Tag Free: Product Information
P43432 (p40) & P43431 (p35)
Human embryonic kidney cell, HEK293-derived mouse IL-12 protein
p40 (Met23-Ser335) & p35 (Arg23-Ala215)
59.9 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. The EC50 for this effect is 0.5-4 pg/mL.
IL12, Mouse, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant mouse IL-12 (Catalog # MF-1012) stimulates cell proliferation of the PHA-activated mouse splenocytes.
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL12, Mouse, HEK293 Cells,Tag Free:Synonyms
IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; cytotoxic lymphocytematuration factor 1, p35)
IL12, Mouse, HEK293 Cells,Tag Free:Background
Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric
pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN- gamma and TNF in
resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the
presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic
potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on murine cells (1).IL-12 is a heterodimeric cytokine composed of a p35 and a p40 subunit. It is produced primarily by antigen-presenting
cells (APCs) and exerts immunoregulatory effects on T and natural killer (NK) cells (2-4).Endogenous IL-12 appears to be a pre-requisite for
generating optimal Th1 responses and plays a pivotal role in promoting cell-mediated immunity against intracellular pathogens (5-7).
1 . Vignali, D.A.et al. (2012 ) Nat Immunol.22-8.
2. Kobayashi M.et al. (1989) J Exp Med 170:827
3. Stern AS. et al. (1990) Proc Natl Acad Sci USA 87:6808
4. Gubler U. et al. (1991) Proc Natl Acad Sci USA 88:4143
5. Wolf SF. et al. (1991) J Immunol 146:3074
6. Cooper AM. et al. (1997) J Exp Med 186:39
7. Mattner F. et al. (1996) Eur J Immunol 26:1553
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