Cat: MF-1003
Cat: MF-1003
IL3, Mouse, HEK293 Cells,Tag Free: Product Information
P01586
Human embryonic kidney cell, HEK293-derived mouse IL-3 protein
Asp33-Cys166
15.1 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The EC50 for this effect is 3-12 pg/mL.
IL3, Mouse, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant mouse IL-3 (Catalog # MF-1003) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cells.
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL3, Mouse, HEK293 Cells,Tag Free:Synonyms
Hematopoietic growth factor; IL3; IL-3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3
IL3, Mouse, HEK293 Cells,Tag Free:Background
Interleukin-3 (IL-3) is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent
hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells,
basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast
cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In
addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and
neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity.
Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.IL-3 exerts its
biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and
beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A
( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha
subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
1. Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
2. Fung, M.C. et al., 1984, Nature 307:233.
3. Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.
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