Cat: HF-1028A
Cat: HF-1028A
IL28A, Human, HEK293 Cells,Tag Free: Product Information
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2
Q8IZJ0
Human embryonic kidney cell, HEK293-derived human IL-28A/IFN-lambda 2 protein Val26-Val200
19.8 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 5-20 ng/mL.
IL28A, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL28A (Catalog # HF -1028A) antivirus activity of the HepG2 human hepatocellular carcinoma cells.
Size-exclusion chromatography of recombinant human IL28A protein (280 nm absorbance)
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL28A, Human, HEK293 Cells,Tag Free:Synonyms
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2
IL28A, Human, HEK293 Cells,Tag Free:Background
Interleukin-28A (IL-28A), IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF -3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Human IL-28A cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide. It shares 94% and 67% aa sequence identity with human IL-28B and human IL-29, respectively.
1. Durbin, R.K. et al. (2013) Immunol. Rev. 255:25.
2. Li, Q. et al. (2013) Front. Biosci. (Landmark Ed.) 18:909.
3. Lopusna, K. et al. (2013) Acta Virol. 57:171.
Human IL33 产品展示
Human IL29 产品展示
Human IL28B 产品展示
Human IL28A 产品展示
Human IL27 产品展示
Mouse CCL20 产品展示
Human IL25 产品展示
Mouse IL25 产品展示
Mouse IL23 产品展示
Human IL21 产品展示
Mouse IL21 产品展示
Human IL17A 产品展示
Mouse IL17A 产品展示
Human IL15 产品展示
Human IL13 产品展示
Mouse IL13 产品展示
Human IL12 产品展示
Mouse IL12 产品展示
Human IL11 产品展示
Mouse IL11 产品展示
Mouse CCL2 产品展示
Human IL10 产品展示
Human IL9 产品展示
Human IL8 产品展示
Human IL7 产品展示
Mouse IL7 产品展示
Human IL6 产品展示
Mouse IL6 产品展示
Human IL5 产品展示
Mouse IL5 产品展示
Human IL4 产品展示
Mouse IL4 产品展示
Human IL3 产品展示
Mouse IL3 产品展示
Human IL1-alpha 产品展示
Mouse IL1-alpha 产品展示
Human IL1-beta 产品展示
Mouse IL1-beta 产品展示
Human IL2 产品展示
Mouse IL2 产品展示
