Cat: HF-1027
Cat: HF-1027
IL27, Human, HEK293 Cells,Tag Free: Product Information
Human IL27; IL-27; IL27; IL-27 subunit alpha; IL27A; IL-27-A; IL27-A; IL27p28; interleukin 27
Q14213.2 (IL-27EBI3) & AAM34498 (IL-27p28)
Human embryonic kidney cell, HEK293-derived human IL-27 protein
IL-27 EBI-3(Arg21-Lys229),IL-27 p28(Phe29-Pro243)
56.0 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 1-6 ng/mL.
IL27, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL27 (Catalog # HF-1027) anti-viral activity using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.
Size-exclusion chromatography of recombinant human IL27 protein (280 nm absorbance)
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL27, Human, HEK293 Cells,Tag Free:Synonyms
Human IL27; IL-27; IL27; IL-27 subunit alpha; IL27A; IL-27-A; IL27-A; IL27p28; interleukin 27
IL27, Human, HEK293 Cells,Tag Free:Background
Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on nave CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).
1. Boulay, J-L. et al. (2003) Immunity 19:159.
2. Trinchieri, G. et al. (2003) Immunity 19:641.
3. Murakami, M. et al. (2004) Growth Factors 22:75.
4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715.
5. Devergne, O. et al. (1996) J. Virology 70:1143.
6. Pflanz, S. et al. (2002) Immunity 16:779.
7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
8. Pflanz, S. et al. (2004) J Immunol 172:2225.
9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.
Human IL33 产品展示
Human IL29 产品展示
Human IL28B 产品展示
Human IL28A 产品展示
Human IL27 产品展示
Mouse CCL20 产品展示
Human IL25 产品展示
Mouse IL25 产品展示
Mouse IL23 产品展示
Human IL21 产品展示
Mouse IL21 产品展示
Human IL17A 产品展示
Mouse IL17A 产品展示
Human IL15 产品展示
Human IL13 产品展示
Mouse IL13 产品展示
Human IL12 产品展示
Mouse IL12 产品展示
Human IL11 产品展示
Mouse IL11 产品展示
Mouse CCL2 产品展示
Human IL10 产品展示
Human IL9 产品展示
Human IL8 产品展示
Human IL7 产品展示
Mouse IL7 产品展示
Human IL6 产品展示
Mouse IL6 产品展示
Human IL5 产品展示
Mouse IL5 产品展示
Human IL4 产品展示
Mouse IL4 产品展示
Human IL3 产品展示
Mouse IL3 产品展示
Human IL1-alpha 产品展示
Mouse IL1-alpha 产品展示
Human IL1-beta 产品展示
Mouse IL1-beta 产品展示
Human IL2 产品展示
Mouse IL2 产品展示
