Cat: HF-1025
Cat: HF-1025
IL25, Human, HEK293 Cells,Tag Free: Product Information
Human IL25; interleukin-25; IL25; IL-25; IL17E; IL-17E; interleukin-17E;
Q9H293
Human embryonic kidney cell, HEK293-derived human IL-25 protein Tyr33-Gly177
16.7 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The EC50 for this effect is 0.2-1.2 ng/mL.
IL25, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL25 (Catalog # HF-1025) induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells.
Size-exclusion chromatography of recombinant human IL25 protein (280 nm absorbance)
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL25, Human, HEK293 Cells,Tag Free:Synonyms
Human IL25; interleukin-25; IL25; IL-25; IL17E; IL-17E; interleukin-17E;
IL25, Human, HEK293 Cells,Tag Free:Background
Interleukin-25 (IL-25) , which is also known as IL-17E, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL-25 shares 80% amino acid sequence identity with mouse and rat IL-25 (3, 4). During helminth infections and allergic reactions, IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4-9). It binds to IL-17 RB but also requires IL-17 RA to exert its activity (3, 8, 10). IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN-gamma, IL-12, IL-23, IL-17A, IL-17F) (4-6, 8, 9, 11-15). Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure (16). Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti-inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti-helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis [12, 14].
1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
2. Iwakura, Y. et al. (2011) Immunity 34:149.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Fort, M.M. et al. (2001) Immunity 15:985.
5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
7, Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
14. Stock, P. et al. (2009) J. Immunol. 182:5116.
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