Cat: HF-1015
IL15, Human, HEK293 Cells,Tag Free: Product Information
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
P40933.1
Human embryonic kidney cell, HEK293-derived human IL-15 proteinAsn49-Ser162
12.8 kDa
Solution protein.
Dissolved in PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is 0.1-1.0 ng/mL.
IL15, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL15 (Catalog # HF-1015) stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells.
Size-exclusion chromatography of recombinant human IL15 protein (280 nm absorbance)
2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL15, Human, HEK293 Cells,Tag Free:Synonyms
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
IL15, Human, HEK293 Cells,Tag Free:Background
Interleukin-15 (IL-15) is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. (1, 2). Mature human IL-15 protein shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of Interleukin 15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). The IL-15 protein binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to Interleukin 15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 protein in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11).
1.De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
2. Grabstein, K. et al. (1994) Science 264:965.
3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
5. Giri, J. et al. (1994) EMBO J. 13:2822.
6. Dubois, S. et al. (2002) Immunity 17:537.
7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
10. Bergamaschi, C. et al. (2012) Blood 120:e1.
11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
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