Cat: HF-1001
Cat: HF-1001
IL1-beta, Human, HEK293 Cells,Tag Free: Product Information
Human IL1 beta; IL-1 beta; IL-1; IL-1b; IL1-BETA;IL1F2; IL-1 beta; interleukin-1 beta P01584
Human embryonic kidney cell, HEK293-derived human IL1-beta protein
Ala117-Ser269
17.4 kDa
Solution protein.
Dissolved in sterile PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
Shipping with dry ice.
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The EC50 for this effect is 1-10 pg/mL.
IL1-beta, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL1-beta (Catalog # HF-1001) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line.
Size-exclusion chromatography of recombinant human IL1-beta protein (280 nm absorbance)
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IL1-beta, Human, HEK293 Cells,Tag Free:Synonyms
Human IL1 beta; IL-1 beta; IL-1; IL-1b; IL1-BETA;IL1F2; IL-1 beta; interleukin-1 beta P01584
IL1-beta, Human, HEK293 Cells,Tag Free:Background
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a name that designates two
pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally
related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to
inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor
and exert identical biological effects. IL-1RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1R accessory protein (IL-1R3/IL-1RAcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1RII has high affinity for IL-1 beta but functions as a decoy receptor
and negative regulator of IL-1 beta activity. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the
cysteine protease IL-1 beta converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.5. March, C.J. et al. (1985) Nature 315:641.
6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
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