Cat: HF-1005
Cat: HF-1005
IL5, Human, HEK293 Cells,Tag Free: Product Information
Human IL5; hIL-5, recombinant IL5, interleukin 5, EDF
P05113
Human embryonic kidney cell, HEK293-derived human IL5 protein
Ile20-Ser134
13 kDa (Monomer)
Dimer in solution
Solution protein
Dissolved in sterile PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The EC50 for this effect is 0.04-0.2 ng/mL.
IL5, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IL5 (Catalog # HF-1005) stimulates cell proliferation of the TF-1 human erythroleukemic cells.
Size-exclusion chromatography of recombinant human IL5 protein (280 nm absorbance)
2 ug/lane protein wasresolved withSDS-PAGEunder non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
IL5, Human, HEK293 Cells,Tag Free:Synonyms
Human IL5; hIL-5, recombinant IL5, interleukin 5, EDF
IL5, Human, HEK293 Cells,Tag Free:Background
Interleukin 5 (IL5), is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 - 3). IL5 is present as a covalently linked antiparallel dimer (4, 5). Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL5, respectively and shows cross-reactivity with mouse IL5. IL5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL2-stimulated invariant natural killer T cells (7, 8). IL5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL5R alpha) and a shared signal-transducing subunit, beta c (3, 6, 11). IL5R alpha first binds IL5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL5R alpha antagonize IL5 and can be found in vivo (10, 14).
1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
3. Martinez-M, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
11. Tavernier, J. et al. (1991) Cell 66:1175.
12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
14. Tavernier, J. et al. (2000) Blood 95:1600.
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