Cat: HF-2016
Cat: HF-2016
IFN-gamma, Human, HEK293 Cells,Tag Free: Product Information
CAA31639
Human embryonic kidney cell, HEK293-derived human IFN-gamma protein
Gln24-Gln166
16.8 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis virus. The EC50 for this effect is 0.10-0.70 ng/mL.
IFN-gamma, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human IFN-gamma (Catalog # HF-2016) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus.
Size-exclusion chromatography of recombinant human IFN-gamma protein (280 nm absorbance)
4ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
IFN-gamma, Human, HEK293 Cells,Tag Free:Synonyms
Human IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma
IFN-gamma, Human, HEK293 Cells,Tag Free:Background
Interon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylatedsubunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%-64% with bovine, canine, equine, feline, and porcine
IFN-gamma, and 37%-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases
the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory
conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction
and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also
exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the
development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of
multiple aspects of atherosclerosis (7).
1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
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