Cat: HF-2004
Cat: HF-2004
GM-CSF/CSF2, Human, HEK293 Cells,Tag Free: Product Information
P04141
Human embryonic kidney cell, HEK293-derived human GM-CSF/CSF2 proteinAla18-Glu144
14.5 kDa
Solution protein.
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 2-20 pg/mL.
GM-CSF/CSF2, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human CSF2 (Catalog # HF-2004) stimulates cell proliferation of the TF-1 human erythroleukemic cells.
Size-exclusion chromatography of recombinant human GM-CSF protein (280 nm absorbance)
2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.
GM-CSF/CSF2, Human, HEK293 Cells,Tag Free:Synonyms
Colony stimulating factor 2, CSF-2, GMCSF
GM-CSF/CSF2, Human, HEK293 Cells,Tag Free:Background
Granulocyte Macrophage Growth Factor(GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent
development. In response to cytokine or inflammatory stimuli, GM-CSF is produced by a number of different cell types, including T cells, B cells,
macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes (1). As a survival factor, GM-CSF activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the
development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF
transfected tumor cells are utilized as cancer vaccines (6, 7). Mature human GM-CSF shares 63%-70% amino acid sequence identity with canine, feline, porcine, and rat GM-CSF and 54% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of
GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and
IL-5 (8, 9). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (10).
1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
8. Onetto-Pothier, N. et al. (1990) Blood 75:59.
9. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
10. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
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