Cat: HF-2008
Cat: HF-2008
HGF, Human, HEK293 Cells,Tag Free: Product Information
DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF;HGF; HGFB; HPTA
P14210
Human embryonic kidney cell, HEK293-derived human HGF protein
Gln32-Ser728
53.7 kDa ( alpha chain) + 26 kDa ( beta chain)
Solution protein
Dissolved in sterile PBS buffer.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C It is recommended that the protein be aliquoted for optimal storage. as supplied.
Shipping with dry ice.
> 95%, determined by SDS-PAGE.
<0.010 EU per 1 ug of the protein by the LAL method.
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The EC50 for this effect is 0.05-0.2 ng/mL.
HGF, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
HGF, Human, HEK293 Cells,Tag Free:Synonyms
DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF;HGF; HGFB; HPTA
HGF, Human, HEK293 Cells,Tag Free:Background
FHepatocyte Growth Factor (HGF) also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
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14. Mineo, R. et al. (1994) Endocrinology 145:4355.
15. Urbanek, K. et al. (2005) Circ. Res. 97:663.