Cat: HF-2027
Cat: HF-2027
R-Spondin1, Human, HEK293 Cells,Tag Free: Product Information
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The EC50 for this effect is 1-10 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).
<0.010 EU per 1 ug of the protein by the LAL method
> 95%, determined by SDS-PAGE
Shipping with dry ice
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Solution protein
Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
25.6 kDa
Human embryonic kidney cell, HEK293-derived human R-Spondin 1 protein
Ser21-Ala263
Q2MKA7
Cristin 3; HRspo1; roof plate-specific spondin; RSPO1; RSpondin 1; R-Spondin 1
R-Spondin1, Human, HEK293 Cells,Tag Free:SDS-PAGE & Bioactivity
Recombinant human R-Spondin 1 (Catalog # HF-2027) induce Topflash reporter activity in human embryonic kidney cells (HEK293T).
2 ug/lane protein wasresolved withSDS-PAGEunder non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
R-Spondin1, Human, HEK293 Cells,Tag Free:Synonyms
Cristin 3; HRspo1; roof plate-specific spondin; RSPO1; RSpondin 1; R-Spondin 1
R-Spondin1, Human, HEK293 Cells,Tag Free:Background
R-Spondin 1 (RSPO1) 12 months from date of receipt, -20 to -70 °C as supplied. , also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). Human R-Spondin 1 (aa21-263) shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, horse, dog, goat, and cow RSPO-1, respectively. R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (8). Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 9). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (10). Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively (11)
1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
8. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
9. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
10. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
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