Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts.
The EC50 for this effect is 4-40 pg/mL.
P29460 (IL-12p40), P29459 (IL-12p35)
Human embryonic kidney cell, HEK293-derived human IL12 protein
L-12 p40 (Ile23-Ser328), IL-12 p35 (Arg23-Ser219)
22.5 kDa + 34.7 kDa
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
2 ug/lane protein wasresolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant human IL12 protein (280 nm absorbance)
Recombinant human IL12 (Catalog # HF-1012) stimulates cell proliferation in PHA-activated human T lymphoblasts.
, , ,:Synonyms
, , ,:Background
