Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.
The EC50 for this effect is 1-10 pg/mL.
NP_000567
Human embryonic kidney cell, HEK293-derived human IL1-beta protein
Ala117-Ser269
17.4 kDa
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
4 ug/lane protein wasresolved withSDS-PAGEunder non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant human IL1-beta protein (280 nm absorbance)
Recombinant human IL1-beta (Catalog # HF-1001) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line.
, , ,:Synonyms
, , ,:Background
