Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.
The EC50 for this effect is 5-40 ng/mL.
Q13253
Human embryonic kidney cell, HEK293-derived human Noggin protein
Gln28-Cys232
23.1 kDa (Monomer)
Disulfide-linked homodimer
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue Staining.
Recombinant human Noggin (Catalog # HF-2019) inhibits BMP-4 induced alkaline phosphatase production in the MC3T3E1mouse preosteoblast cells.
, , ,:Synonyms
, , ,:Background
