Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.
The EC50 for this effect is 0.01-0.1 ng/mL.
P09038
Human embryonic kidney cell, HEK293-derived human FGF basic/FGF2/bFGF protein
Met134-Ser288
16.5 kDa
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
2 ug/lane protein wasresolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant human FGF basic/FGF2 protein (280 nm absorbance)
Recombinant human FGF basic/FGF2(Catalog# HF-2018) stimulates cell proliferation of the NRR-3T3 human erythroleukemic cells.
, , ,:Synonyms
, , ,:Background
