Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
The EC50 for this effect is 0.05-0.2 ng/mL.
P14210
Human embryonic kidney cell, HEK293-derived human HGF protein
Gln32-Ser728
53.7 kDa ( alpha chain) + 26 kDa ( beta chain)
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
4 ug/lane protein wasresolved with SDS-PAGE undernon-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant human HGF protein (280 nm absorbance)
Recombinant human HGF (Catalog # HF-2008) induces IL11 secretion by Saos-2 human osteosarcoma cells.
, , ,:Synonyms
, , ,:Background
